Application

Immunoblotting (1 µg/ml, non-reducing, see comments)r>Immunofluorescence (see comments)r>Immunohistochemistry (2-5 µg/ml)r>Immunoprecipitation (see comments)

General description

Recognizes the ~300 kDa mannose 6-phosphate receptor protein.

Purified mouse monoclonal antibody. Recognizes the mannose 6-phosphate receptor protein.

This Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) is validated for use in Immunoblotting, IF, IHC, IP for the detection of Mannose 6-Phosphate Receptor.

Immunogen

native bovine cation independent (CI)-mannose 6-phosphate receptor

Epitope: within the extracelluar domain

Bovine

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.

Dintzis, S., et al. 1994. J. Biol. Chem.269, 12159.Dintzis, S., and Pfeffer, S. 1990. EMBO J.9, 77.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)